• 其他栏目

    吴尚犬

    • 特任教授 博士生导师 硕士生导师
    • 教师拼音名称:Wu Shangquan
    • 电子邮箱:
    • 学历:研究生(博士)毕业
    • 学位:博士
    • 毕业院校:中国科学技术大学
    • 学科:力学
    • 2022当选:国家优秀青年基金获得者

    访问量:

    开通时间:..

    最后更新时间:..

    Quantifying 3D cell-matrix interactions during mitosis and the effect of anticancer drugs on the interactions

    点击次数:

    影响因子:8.9

    DOI码:10.1007/s12274-021-3357-4

    发表刊物:Nano Research

    关键字:cell division traction force paclitaxel nocodazole

    摘要:The mechanical force between cells and the extracellular microenvironment is crucial to many physiological processes such as cancer metastasis and stem cell differentiation. Mitosis plays an essential role in all these processes and thus an in-depth understanding of forces during mitosis gains insight into disease diagnosis and disease treatment. Here, we develop a traction force microscope method based on monolayer fluorescent beads for measuring the weak traction force (tens of Pa) of mitotic cells in three dimensions. We quantify traction forces of human ovarian granulosa (KGN) cells exerted on the extracellular matrix throughout the entire cell cycle in three dimensions. Our measurements reveal how forces vary during the cell cycle, especially during cell division. Furthermore, we study the effect of paclitaxel (PTX) and nocodazole (NDZ) on mitotic KGN cells through the measurement of traction forces. Our results show that mitotic cells with high concentrations of PTX exert a larger force than those with high concentrations of NDZ, which proved to be caused by changes in the structure and number of microtubules. These findings reveal the key functions of microtubule in generating traction forces during cell mitosis and explain how dividing cells regulate themselves in response to anti-mitosis drugs. This work provides a powerful tool for investigating cell-matrix interactions during mitosis and may offer a potential way to new therapies for cancer.

    合写作者:Su Yong,Xu Xiaohai,Liu Hong,Mei Kainan,Lan Shihai,Zhang Shubo,Wu Xiaoping

    第一作者:Liu Yongman,Wang Jianye

    论文类型:期刊论文

    通讯作者:Wu Shangquan*,Zhang Qingchuan*,Cao Yunxia*

    卷号:14

    页面范围:4163–4172

    是否译文:

    发表时间:2021-03-03

    收录刊物:SCI