点击次数：

影响因子：10.6

DOI码：10.1016/j.actbio.2021.11.001

发表刊物：Acta Biomaterialia

关键字：magnetic nanocomposite hydrogel fe3o4 magnetic nanoparticles matrix stiffening human mesenchymal stem cells morphology differentiation elasticity ph mechanotransduction mobility surface ligand

摘要：As cells have the capacity to respond to their mechanical environment, cellular biological behaviors can be regulated by the stiffness of extracellular matrix. Moreover, biological processes are dynamic and accompanied by matrix stiffening. Herein, we developed a stiffening cell culture platform based on polyacrylamide-Fe3O4 magnetic nanocomposite hydrogel with tunable stiffness under the application of magnetic field. This platform provided a wide range of tunable stiffness (-0.3-20 kPa) covering most of human tissue elasticity with a high biocompatibility. Overall, the increased magnetic interactions between magnetic nanoparticles reduced the pore size of the hydrogel and enhanced the hydrogel stiffness, thereby facilitating the adhesion and spreading of stem cells, which was attributed to the F-actin assembly and vinculin recruitment. Such stiffening cell culture platform provides dynamic mechanical environments for probing the cellular response to matrix stiffening, and benefits studies of dynamic biological processes.

合写作者：Rao Depeng,Chen Ye,Wang Yu

第一作者：Yan Tianhao

论文类型：期刊论文

通讯作者：Zhang Qingchuan*,Wu Shangquan*

卷号：138

页面范围：112-123

是否译文：否

发表时间：2022-05-01

收录刊物：SCI

发布期刊链接：https://www.sciencedirect.com/science/article/pii/S174270612100742X