Impact Factor: 7.46

DOI number: 10.1016/j.snb.2015.11.092

Journal: Sensors and Actuators B-Chemical

Key Words: microcantilever nanomechanical sensor aflatoxin b-1 label-free surface stress liquid-chromatography assay food mycotoxins biomarkers biosensors exposure antigen

Abstract: Aflatoxin B-1 (AFB(1)), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB(1) are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB(1) with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB(1) antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB(1) concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB(1), which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB(1) dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB(1) and may have great potential for food quality control and public health protection. (C) 2015 Elsevier B.V. All rights reserved.

Indexed by: Journal paper

Volume: 226

Page Number: 24-29

Translation or Not: no

Date of Publication: 2016-04-01

Links to published journals: https://www.sciencedirect.com/science/article/pii/S0925400515306742