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影响因子：7.0

DOI码：10.1021/ac404065m

发表刊物：Analytical Chemistry

关键字：*Biosensing Techniques Immunoglobulin Fragments/*immunology

摘要：The improvement of sensitivity is of great significance to the application of biochemical sensor. In this study, we propose a micocantilever-based immunosensor in surface stress mode using half antibody fragments as receptor molecules. The thiol-containing half antibody fragment was obtained with a low loss of antibody biological activity and then was covalently and orientedly immobilized on the gold surface of microcantilevers via two native thiol groups. Such a one-step reaction and immobilization of receptor molecule simplify the preparation process of micocantilever immunosensor. Using shortened and highly oriented half antibody fragments as receptor molecules, the generation of surface stress and the transmission of stress from the interaction region of molecules to the surface of the microcantilever have been elevated significantly. The limit of detection (LOD) of the presented sensor has been significantly lowered to 1 pg/mL, or 1.1 pM in equivalence, which is a 500-fold improvement when compared with intact full antibody coated conventional micocantilever sensors. The results indicate that the half antibody fragment is well suited for the functionalization of the microcantilever surface and is generally applicable to all microcantilever immunosensor development, and this principle will help to design a functional film of devices with significantly lower LOD.

合写作者：Wu Xiaoping,Liang Xin

第一作者：Wu Shangquan

论文类型：期刊论文

通讯作者：Zhang Qingchuan,Wang Baomin,Liu Hong

卷号：86

页面范围：4271-4277

是否译文：否

发表时间：2014-05-06

收录刊物：SCI