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影响因子：6.9

DOI码：10.1016/j.aca.2018.02.072

发表刊物：Analytica Chimica Acta

关键字：Aptamers, Nucleotide/*chemistry *Biosensing Techniques Insecticides/*analysis Organothiophosphates/*analysis Aptamer Label-free Microcantilever sensor Profenofos Surface stress

摘要：Profenofos, a highly poisonous organophosphorus pesticide, has been widely used in agricultural production. These pesticide residues have seriously influenced food security and threatened human health, and new methods with high sensitivity are greatly needed to detect profenofos. Here, we developed an aptamer-based microcantilever-array sensor operated in stress mode to detect profenofos, with advantages of being a label-free, highly sensitive, one-step immobilization method capable of quantitative and real-time detection. The microcantilevers were functionalized with a profenofos-specific aptamer (SS2-55), and then the specific binding of profenofos to aptamer induced a deflection of the microcantilever, which was monitored using an optical method in a real-time manner. The microcantilever deflection showed a positive relationship with profenofos concentration, and the detection limit was low to 1.3ngmL(-1) (3.5nM) for profenofos, which was much lower than other aptamer-based detection methods. The selectivity of the sensor was verified with another organophosphorus pesticide. Additionally, we successfully detected profenofos dissolved in vegetable-soak solution. Our results showed that this aptamer-based microcantilever-array sensor is a convenient and label-free method for detecting profenofos in small amounts and has great potential for food-security applications.

合写作者：Zhang Guangping

第一作者：Li Chao

论文类型：期刊论文

通讯作者：Wu Shangquan*,Qingchuan Zhang*

卷号：1020

页面范围：116-122

是否译文：否

发表时间：2018-08-22