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影响因子：7.46

DOI码：10.1016/j.snb.2015.11.092

发表刊物：Sensors and Actuators B-Chemical

关键字：microcantilever nanomechanical sensor aflatoxin b-1 label-free surface stress liquid-chromatography assay food mycotoxins biomarkers biosensors exposure antigen

摘要：Aflatoxin B-1 (AFB(1)), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB(1) are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB(1) with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB(1) antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB(1) concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB(1), which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB(1) dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB(1) and may have great potential for food quality control and public health protection. (C) 2015 Elsevier B.V. All rights reserved.

论文类型：期刊论文

卷号：226

页面范围：24-29

是否译文：否

发表时间：2016-04-01

发布期刊链接：https://www.sciencedirect.com/science/article/pii/S0925400515306742