点击次数：

影响因子：10.3

DOI码：10.1007/s12274-021-3588-4

发表刊物：Nano Research

关键字：adsorption-induced inactivation early cancer detection nanobody-based biosensor stress enhancement tumor markers

摘要：Early cancer diagnosis requires ultrasensitive detection of tumor markers in blood. To this end, we develop a novel microcantilever immunosensor using nanobodies (Nbs) as receptors. As the smallest antibody (Ab) entity comprising an intact antigen-binding site, Nbs achieve dense receptor layers and short distances between antigen-binding regions and sensor surfaces, which significantly elevate the generation and transmission of surface stress. Owing to the inherent thiol group at the C-terminus, Nbs are covalently immobilized on microcantilever surfaces in directed orientation via one-step reaction, which further enhances the stress generation. For microcantilever-based nanomechanical sensor, these advantages dramatically increase the sensor sensitivity. Thus, Nb-functionalized microcantilevers can detect picomolar concentrations of tumor markers with three orders of magnitude higher sensitivity, when compared with conventional Ab-functionalized microcantilevers. This proof-of-concept study demonstrates an ultrasensitive, label-free, rapid, and low-cost method for tumor marker detection. Moreover, interestingly, we find Nb inactivation on sensor interfaces when using macromolecule blocking reagents. The adsorption-induced inactivation is presumably caused by the change of interfacial properties, due to binding site occlusion upon complex coimmobilization formations. Our findings are generalized to any coimmobilization methodology for Nbs and, thus, for the construction of high-performance immuno-surfaces.

第一作者：Rao Depeng,Mei Kainan

论文类型：期刊论文

通讯作者：Zhang Qingchuan*,Wu Shangquan*

卷号：15

页面范围：1003-1012

是否译文：否

发表时间：2022-02-01

收录刊物：SCI

发布期刊链接：https://link.springer.com/article/10.1007/s12274-021-3588-4