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影响因子：5.6

发表刊物：APL Photonics

摘要：Morphology and motility are essential criteria for assessing sperm viability. However, the human sperm head is small (∼ 3–4 μm) and requires a relatively high-magnification microscope objective, while the sperm flagella (∼ 45 μm) are poorly visible with complex 3D properties. Microscopic dynamic observation of intact sperm in 3D is challenging. Conventional inspection methods with a limited depth of field are inadequate for this issue. To provide a solution to this critical need, we develop pixelated polarization-based parallel phase-shifting digital holographic microscopy for the 3D dynamic observation of human sperm. Compared to conventional holographic imaging, this approach can effectively separate the object wavefront and avoid image quality degradation while fully exploiting the spatial bandwidth of the camera. We propose the use of the Stokes parameter reconstruction method to reconstruct the object …

合写作者：赵江成,姜兆祥

第一作者：白传彪

论文类型：期刊论文

通讯作者：吴尚犬,张青川

卷号：8

期号：7

页面范围：076108

是否译文：否

发表时间：2023-07-01

发布期刊链接：https://pubs.aip.org/aip/app/article/8/7/076108/2902244/3D-dynamic-observation-of-human-sperm-by-parallel