Cui Hua
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- Professor
- Supervisor of Doctorate Candidates
- Supervisor of Master's Candidates
- Name (English):Hua Cui
- Name (Pinyin):Cui Hua
- E-Mail:
- Business Address:环境资源楼-339
- Contact Information:0551－3600730
- Degree:Dr
- Professional Title:Professor
- Teacher College:Chemistry and Materials Science
Contact Information
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- Paper Publications
Ultrasensitive electrochemiluminescence immunosensor based on luminol functionalized gold nanoparticle labeling
Release time:2021-08-02 Hits:
- DOI number:10.1016/j.bios.2010.03.014
- Journal:Biosens. Bioelectron.
- Abstract:An ultrasensitive electrochemiluminescence (ECL) immunosensor based on luminol functionalized gold nanoparticle (AuNP) labeling was developed using human immunoglobulin G (hIgG) as a model analyte. The primary antibody biotin-conjugated goat-anti-human IgG was first immobilized on a streptavidin coated AuNP modified electrode, then the antigen (human IgG) and the luminol functionalized AuNP-labeled second antibody were conjugated successively to form a sandwich-type immunocomplex, i.e. immunosensor. ECL was carried out with a double-step potential in carbonate buffer solution containing 1.0 mmol/L H2O2. Since thousand of luminol molecules were coated on the surface of AuNPs to realize labeling of multiple molecules with CL activity at a single antibody and the amplification of AuNPs and biotin–streptavidin system was utilized, luminol ECL signal could be enhanced greatly, finally resulting in extremely high sensitivity. The ECL method shows a detection limit of 1.0 pg/mL (S/N = 3) for hIgG, which is superior to all previously reported methods for the determination of hIgG. Moreover, the proposed method is also simple, stable, specific, and time-saving, avoiding the complicated stripping procedure during CL detection and the uncontrollable synthesis of irregular nanoparticles compared with other chemiluminescence immunoassay based on AuNP labeling. Additionally, the labeling procedure is also superior to that of other reported multilabeling strategies, such as Ru complex-encapsulated polymer microspheres, and most of Ru complex-encapsulated liposomes in simplicity, stability, labeling property and practical applicability. Finally, the proposed method has been successfully applied to the detection of hIgG in human serums.
- Co-author:段春凤,王伟
- First Author:田大勇
- Indexed by:Journal paper
- Correspondence Author:Hua Cui
- Discipline:Natural Science
- Document Type:J
- Volume:25
- Issue:10
- Page Number:2290-2295
- Translation or Not:no
- Date of Publication:2010-06-15
- Included Journals:SCI
- Links to published journals:https://www.sciencedirect.com/science/article/pii/S0956566310001326?via%3Dihub