A homogeneous hemin/G-quadruplex DNAzyme based turn-on chemiluminescence aptasensor for interferon-gamma detection via in-situ assembly of luminol functionalized gold nanoparticles, deoxyribonucleic acid, interferon-gamma and hemin

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• Impact Factor:5.977
• DOI number:10.1016/j.aca.2013.06.048
• Journal:Anal. Chim. Acta
• Abstract:A homogeneous hemin/G-quadruplex DNAzyme (HGDNAzyme) based turn-on chemiluminescence aptasensor for interferon-gamma (IFN-γ) detection is developed, via dynamic in-situ assembly of luminol functionalized gold nanoparticles (lum-AuNPs), DNA, IFN-γ and hemin. The G-quadruplex oligomer of the HGDNAzyme was split into two halves, which was connected with the complementary sequence of P1 (IFN-γ-binding aptamer) to form the oligonucleotide P2. P2 hybridized with IFN-γ-binding aptamer and meanwhile assembled onto lum-AuNPs through biotin–streptavidin specific interaction. When IFN-γ was recognized by aptamer, P2 was released into the solution. The two lateral portions of P2 combined with hemin to yield the catalytic hemin/G-quadruplex DNAzyme, which amplified the luminol oxidation for a turn-on chemiluminescence signaling. Based on this strategy, the homogeneous aptasensor enables the facile detection of IFN-γ in a range of 0.5–100 nM. Moreover, the aptasensor showed high sensitivity (0.4 nM) and satisfactory specificity, pointing to great potential applications in clinical analysis.
• Co-author:何毅,于秀霞
• First Author:蒋杰
• Indexed by:Journal paper
• Correspondence Author:Hua Cui
• Document Code:000323085600007
• Discipline:Natural Science
• Document Type:J
• Volume:791
• Page Number:60-64
• ISSN No.:0003-2670
• Translation or Not:no
• Date of Publication:2013-08-12
• Included Journals:SCI
• Links to published journals:https://www.sciencedirect.com/science/article/pii/S0003267013008921