Dynamically Tunable Chemiluminescence of Luminol-Functionalized Silver Nanoparticles and Its Application to Protein Sensing Arrays

Release time:2021-07-23  Hits:

• Impact Factor:6.785
• DOI number:10.1021/ac503123q
• Journal:Anal. Chem.
• Abstract:It is still a great challenge to develop an array-based sensing system that can obtain only multiparameters, according to a single experiment and device. The role of conventional chemiluminescence (CL) in biosensing has been limited to a signal transducer in which a single signal (CL intensity) can be obtained for quantifying the concentrations of analytes. In this work, we have developed an dynamically tunable CL system, based on the reaction of luminol-functionalized silver nanoparticles (luminol–AgNPs) with H2O2, which could be tunable via adjusting various conditions such as the concentration of H2O2, pH value, and addition of protein. A single experiment operation could obtain multiparameters including CL intensity, the time to appear CL emission and the time to reach CL peak value. The tunable, low-background, and highly reproducible CL system based on luminol–AgNPs is applied, for the first time, as a sensing platform with trichannel properties for protein sensing arrays by principal component analysis. Identification of 35 unknowns demonstrated a success rate of >96%. The developed sensing arrays based on the luminol–AgNPs provide a new way to use nanoparticles-based CL for the fabrication of sensing arrays and hold great promise for biomedical application in the future.
• Co-author:何潇,刘晓英,高凌峰
• First Author:何毅
• Indexed by:Journal paper
• Correspondence Author:Hua Cui
• Document Code:000346683900037
• Discipline:Natural Science
• Document Type:J
• Volume:86
• Issue:24
• Page Number:12166-12171
• ISSN No.:0003-2700
• Translation or Not:no
• Date of Publication:2014-12-16
• Included Journals:SCI
• Links to published journals:https://pubs.acs.org/doi/10.1021/ac503123q