Cui Hua
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- Professor
- Supervisor of Doctorate Candidates
- Supervisor of Master's Candidates
- Name (English):Hua Cui
- Name (Pinyin):Cui Hua
- E-Mail:
- Business Address:环境资源楼-339
- Contact Information:0551－3600730
- Degree:Dr
- Professional Title:Professor
- Teacher College:Chemistry and Materials Science
Contact Information
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- Paper Publications
Quenching the Chemiluminescence of Acridinium Ester by Graphene Oxide for Label-Free and Homogeneous DNA Detection
Release time:2021-07-23 Hits:
- DOI number:10.1021/am404138x
- Journal:ACS Appl. Mater. Interfaces
- Abstract:It was found that graphene oxide (GO) could effectively quench the chemiluminescence (CL) emission from a acridinium ester (AE)-hydrogen peroxide system. By taking advantage of this quenching effect, as a proof of concept, a label-free and homogeneous DNA assay was developed for the detection of Mycobacterium tuberculosis DNA. In the absence of target DNA, both probe DNA and AE were absorbed on the surface of GO, producing a weak CL emission owing to the CL quenching effect of GO. However, in the presence of target DNA, a double-stranded structure of DNA was generated, leading to the release of the oligonucleotide from the GO surface. AE favors binding with double-stranded DNA, which will be released from the GO surface; thus, the quenching effect of GO will be no longer effective and a strong CL signal can be observed. This assay can detect M. tuberculosis DNA with a detection limit of 0.65 nM. This sensitivity is lower than that of previously reported electrochemical detection.
- Co-author:huangguangming
- First Author:何毅
- Indexed by:Journal paper
- Correspondence Author:Hua Cui
- Document Code:000327103500122
- Discipline:Natural Science
- Document Type:J
- Volume:5
- Issue:21
- Page Number:11336-11340
- ISSN No.:1944-8244
- Translation or Not:no
- Date of Publication:2013-11-13
- Included Journals:SCI
- Links to published journals:https://pubs.acs.org/doi/10.1021/am404138x
