Cui Hua
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- Professor
- Supervisor of Doctorate Candidates
- Supervisor of Master's Candidates
- Name (English):Hua Cui
- Name (Pinyin):Cui Hua
- E-Mail:
- Business Address:环境资源楼-339
- Contact Information:0551－3600730
- Degree:Dr
- Professional Title:Professor
- Teacher College:Chemistry and Materials Science
Contact Information
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- Paper Publications
Sensitive Immunosensor for N-Terminal Pro-brain Natriuretic Peptide Based on N-(Aminobutyl)-N-(ethylisoluminol)-Functionalized Gold Nanodots/Multiwalled Carbon Nanotube Electrochemiluminescence Nanointerface
Release time:2021-07-23 Hits:
- DOI number:10.1021/am509094p
- Journal:ACS Appl. Mater. Interfaces
- Abstract:A novel electrochemiluminescence (ECL) immunosensor was developed for the determination of N-terminal pro-brain natriuretic peptide (NT-proBNP) by using N-(aminobutyl)-N-(ethylisoluminol) (ABEI)-functionalized gold nanodots/chitosan/multiwalled carbon nanotubes (ABEI/GNDs/chitosan/COOH-MWCNTs) hybrid as nanointerface. First, ABEI/GNDs/chitosan/COOH-MWCNTs hybrid nanomaterials were grafted onto the surface of ITO electrode via the film-forming property of hybrid nanomaterials. The anti-NT-proBNP antibody was connected to the surface of modified electrode by virtue of amide reaction via glutaraldehyde. The obtained sensing platform showed strong and stable ECL signal. When NT-proBNP was captured by its antibody immobilized on the sensing platform via immunoreaction, the ECL intensity decreased. Direct ECL signal changes were used for the determination of NT-proBNP. The present ECL immunosensor demonstrated a quite wide linear range of 0.01–100 pg/mL. The achieved low detection limit of 3.86 fg/mL was about 3 orders of magnitude lower than that obtained with electrochemistry method reported previously. Because of the simple and fast analysis, high sensitivity and selectivity, and stable and reliable response, the present immunosensor has been successfully applied to quantify NT-proBNP in practical plasma samples. The success of the sensor in this work also confirms that ABEI/GNDs/chitosan/COOH-MWCNTs hybrid is an ideal nanointerface to fabricate a sensing platform. Furthermore, the proposed strategy could be applied in the detection of other clinically important biomarkers.
- Co-author:韩智莉,李芳,杨迪,卞智萍
- First Author:张宏丽
- Indexed by:Journal paper
- Correspondence Author:Hua Cui
- Document Code:000353177000021
- Discipline:Natural Science
- Document Type:J
- Volume:7
- Issue:14
- Page Number:7599-7604
- ISSN No.:1944-8244
- Translation or Not:no
- Date of Publication:2015-04-15
- Included Journals:SCI
- Links to published journals:https://pubs.acs.org/doi/10.1021/am509094p
