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DOI码：10.1016/j.bios.2010.03.014

发表刊物：Biosens. Bioelectron.

摘要：An ultrasensitive electrochemiluminescence (ECL) immunosensor based on luminol functionalized gold nanoparticle (AuNP) labeling was developed using human immunoglobulin G (hIgG) as a model analyte. The primary antibody biotin-conjugated goat-anti-human IgG was first immobilized on a streptavidin coated AuNP modified electrode, then the antigen (human IgG) and the luminol functionalized AuNP-labeled second antibody were conjugated successively to form a sandwich-type immunocomplex, i.e. immunosensor. ECL was carried out with a double-step potential in carbonate buffer solution containing 1.0 mmol/L H2O2. Since thousand of luminol molecules were coated on the surface of AuNPs to realize labeling of multiple molecules with CL activity at a single antibody and the amplification of AuNPs and biotin–streptavidin system was utilized, luminol ECL signal could be enhanced greatly, finally resulting in extremely high sensitivity. The ECL method shows a detection limit of 1.0 pg/mL (S/N = 3) for hIgG, which is superior to all previously reported methods for the determination of hIgG. Moreover, the proposed method is also simple, stable, specific, and time-saving, avoiding the complicated stripping procedure during CL detection and the uncontrollable synthesis of irregular nanoparticles compared with other chemiluminescence immunoassay based on AuNP labeling. Additionally, the labeling procedure is also superior to that of other reported multilabeling strategies, such as Ru complex-encapsulated polymer microspheres, and most of Ru complex-encapsulated liposomes in simplicity, stability, labeling property and practical applicability. Finally, the proposed method has been successfully applied to the detection of hIgG in human serums.

合写作者：段春凤,王伟

第一作者：田大勇

论文类型：期刊论文

通讯作者：崔华

学科门类：理学

文献类型：J

卷号：25

期号：10

页面范围：2290-2295

是否译文：否

发表时间：2010-06-15

收录刊物：SCI

发布期刊链接：https://www.sciencedirect.com/science/article/pii/S0956566310001326?via%3Dihub