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影响因子：5.977

DOI码：10.1016/j.aca.2013.06.048

发表刊物：Anal. Chim. Acta

摘要：A homogeneous hemin/G-quadruplex DNAzyme (HGDNAzyme) based turn-on chemiluminescence aptasensor for interferon-gamma (IFN-γ) detection is developed, via dynamic in-situ assembly of luminol functionalized gold nanoparticles (lum-AuNPs), DNA, IFN-γ and hemin. The G-quadruplex oligomer of the HGDNAzyme was split into two halves, which was connected with the complementary sequence of P1 (IFN-γ-binding aptamer) to form the oligonucleotide P2. P2 hybridized with IFN-γ-binding aptamer and meanwhile assembled onto lum-AuNPs through biotin–streptavidin specific interaction. When IFN-γ was recognized by aptamer, P2 was released into the solution. The two lateral portions of P2 combined with hemin to yield the catalytic hemin/G-quadruplex DNAzyme, which amplified the luminol oxidation for a turn-on chemiluminescence signaling. Based on this strategy, the homogeneous aptasensor enables the facile detection of IFN-γ in a range of 0.5–100 nM. Moreover, the aptasensor showed high sensitivity (0.4 nM) and satisfactory specificity, pointing to great potential applications in clinical analysis.

合写作者：何毅,于秀霞

第一作者：蒋杰

论文类型：期刊论文

通讯作者：崔华

论文编号：000323085600007

学科门类：理学

文献类型：J

卷号：791

页面范围：60-64

ISSN号：0003-2670

是否译文：否

发表时间：2013-08-12

收录刊物：SCI

发布期刊链接：https://www.sciencedirect.com/science/article/pii/S0003267013008921