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DOI码：10.1021/am404138x

发表刊物：ACS Appl. Mater. Interfaces

摘要：It was found that graphene oxide (GO) could effectively quench the chemiluminescence (CL) emission from a acridinium ester (AE)-hydrogen peroxide system. By taking advantage of this quenching effect, as a proof of concept, a label-free and homogeneous DNA assay was developed for the detection of Mycobacterium tuberculosis DNA. In the absence of target DNA, both probe DNA and AE were absorbed on the surface of GO, producing a weak CL emission owing to the CL quenching effect of GO. However, in the presence of target DNA, a double-stranded structure of DNA was generated, leading to the release of the oligonucleotide from the GO surface. AE favors binding with double-stranded DNA, which will be released from the GO surface; thus, the quenching effect of GO will be no longer effective and a strong CL signal can be observed. This assay can detect M. tuberculosis DNA with a detection limit of 0.65 nM. This sensitivity is lower than that of previously reported electrochemical detection.

合写作者：黄光明

第一作者：何毅

论文类型：期刊论文

通讯作者：崔华

论文编号：000327103500122

学科门类：理学

文献类型：J

卷号：5

期号：21

页面范围：11336-11340

ISSN号：1944-8244

是否译文：否

发表时间：2013-11-13

收录刊物：SCI

发布期刊链接：https://pubs.acs.org/doi/10.1021/am404138x