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DOI码：10.1021/acs.analchem.5c00462

发表刊物：Analytical Chemistry

摘要：In this study, a novel chemiluminescence (CL)-functionalized nanomaterial, designated as ABEI@Cu2O nanocubes, was synthesized by doping the CL reagent N-(4-aminobutyl)-N-ethylisoluminol (ABEI) into Cu2O nanocubes via a simple one-pot method. The Cu2O nanocubes not only functioned as an effective nanocarrier but also exhibited peroxidase-like activity, promoting the decomposition of H2O2 to enhance CL emission. The incorporation of ABEI into the Cu2O nanocubes resulted in the concentration of the CL reagent within the cubic structures, reducing the spatial distance between the catalyst and ABEI and, thereby, enhancing the CL signal. The robust and stable CL emission of ABEI@Cu2O nanocubes facilitated the development of a label-free CL immunoassay for the detection of cardiac troponin I (cTnI), a biomarker indicative of an acute myocardial infarction. This immunoassay demonstrated a wide linear detection range from 1.0 × 10–13 to 1.0 × 10–8 g/mL and a low detection limit of 3.27 × 10–14 g/mL, surpassing all existing CL immunoassays for cTnI detection. Moreover, the proposed immunoassay demonstrated excellent sensitivity and precision in the quantitative detection of cTnI in serum samples from both healthy individuals and patients. The exceptional sensitivity and flexibility of this immunoassay, supported by the unique properties of the ABEI@Cu2O nanocubes, highlight its significant potential for the detection of various biomarkers.

第一作者：张文灿

合写作者：王慢莉,郑可颖

论文类型：期刊论文

通讯作者：舒江南,崔华

学科门类：理学

文献类型：J

卷号：97

期号：23

页面范围：12116–12124

是否译文：否

发表时间：2025-06-04

收录刊物：SCI

发布期刊链接：https://pubs.acs.org/doi/10.1021/acs.analchem.5c00462?ref=PDF