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    崔华

    • 教授 博士生导师 硕士生导师
    • 教师英文名称:Hua Cui
    • 电子邮箱:
    • 办公地点:环境资源楼-339
    • 联系方式:0551-3600730
    • 学位:博士
    • 2006当选:国家杰出青年科学基金获得者

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    A general strategy to prepare homogeneous and reagentless GO/lucigenin&enzyme biosensors for detection of small biomolecules

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    影响因子:10.257

    DOI码:10.1016/j.bios.2014.01.057

    发表刊物:Biosens. Bioelectron.

    摘要:In this work, a novel biosensor was developed for the detection of glucose based on glucose oxidase (GOD) functionalized graphene oxide (GO)/lucigenin nanocomposite. In this sensing strategy, GO/lucigenin composite was first prepared by vigorously stirring GO with lucigenin. Then the functionalization of GOD was achieved by simply storing GOD with GO/lucigenin at 4 °C overnight to form GO/lucigenin&GOD composite. When glucose was incubated with GO/lucigenin&GOD composite for 50 min to generate H2O2, followed by the injection of 0.2 M NaOH, CL signal was detected due to the reaction of lucigenin with H2O2. Glucose could be determined in the range of 1.0×10−6–5.0×10−3 g mL−1 with a detection limit of 9.9×10−7 g mL−1. The present biosensor has been successfully applied for the detection of glucose in human serum samples. Compared with previously reported methods, this sensing strategy is homogeneous and reagentless and avoids complicated assembly procedure and pretreatment of serum sample, showing good stability, repeatability, high selectivity and simplicity. Moreover, this strategy has been demonstrated to be a general strategy by replacing GOD with other enzymes such as uricase and choline oxidase for the detection of small molecules such as uric acid and choline. The proposed biosensors may find future applications in the fields such as disease diagnosis and biomedicine.

    第一作者:高凌峰

    合写作者:张宏丽

    论文类型:期刊论文

    通讯作者:崔华

    论文编号:000334086100011

    学科门类:理学

    文献类型:J

    卷号:57

    页面范围:65-70

    ISSN号:0956-5663

    是否译文:

    发表时间:2014-07-15

    收录刊物:SCI

    发布期刊链接:https://www.sciencedirect.com/science/article/pii/S095656631400075X