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DOI码：10.1021/acsami.5b05325

发表刊物：ACS Appl. Mater. Interfaces

摘要：N-aminobutyl-N-ethylisoluminol and horseradish peroxidase bifunctionalized graphene oxide hybrids (ABEI-GO@HRP) were prepared through a facile and green strategy for the first time. The hybrids exhibited excellent chemiluminescence (CL) activity over a wide range of pH from 6.1 to 13.0 when reacted with H2O2, whereas ABEI functionalized GO had no CL emission at neutral pH and showed more than 2 orders of magnitude lower CL intensity than ABEI-GO@HRP at pH 13.0. Such strong CL emission from ABEI-GO@HRP was probably due to that HRP and GO facilitated the formation of O2•–, – CO4•2–, HO•, and π-C═C• in the CL reaction, and GO as a reaction interface promoted the electron transfer of the radical-involved reaction. By virtue of ABEI-GO@HRP as a platform, an ultrasensitive, selective, and reagentless CL sensor was developed for H2O2 detection. The CL sensor exhibited a detection limit of 47 fM at physiological pH, which was more than 2 orders of magnitude lower than previously reported methods. This work reveals that bifunctionalization of GO by ABEI and HRP leads to excellent CL feature and enzyme selectivity, which can be used as an ideal platform for developing novel analytical methods.

第一作者：刘晓英

合写作者：韩智莉,李芳,高凌峰,梁高林

论文类型：期刊论文

通讯作者：崔华

论文编号：000360322000013

学科门类：理学

文献类型：J

卷号：7

期号：33

页面范围：18283-18291

ISSN号：1944-8244

是否译文：否

发表时间：2015-08-26

收录刊物：SCI

发布期刊链接：https://pubs.acs.org/doi/10.1021/acsami.5b05325