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    宋晓元

    • 教授 博士生导师 硕士生导师
    • 教师英文名称:Xiaoyuan Song
    • 教师拼音名称:songxiaoyuan
    • 电子邮箱:
    • 学历:博士研究生毕业
    • 办公地点:中国科学技术大学西校区科技东楼915
    • 学位:博士
    • 毕业院校:美国罗彻斯特大学
    • 学科:生物学
    • 2022-10-01曾获荣誉当选:“典赞 2022科普安徽”科普活动“年度科普作品”
    • 2021-05-01曾获荣誉当选:安徽省优秀科普作品一等奖
    • 2019-09-16曾获荣誉当选:王宽诚育才奖

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    Rearrangement of macronucleus chromosomes correspond to TAD-like structures of micronucleus chromosomes in Tetrahymena thermophila.

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    DOI码:10.1101/gr.241687.118

    发表刊物:Genome Research

    摘要:The somatic macronucleus (MAC) and germline micronucleus (MIC) of Tetrahymena thermophila differ in chromosome numbers, sizes, functions, transcriptional activities, and cohesin complex location. However, the higher-order chromatin organization in T. thermophila is still largely unknown. Here, we explored the higher-order chromatin organization in the two distinct nuclei of T. thermophila using the Hi-C and HiChIP methods. We found that the meiotic crescent MIC has a specific chromosome interaction pattern, with all the telomeres or centromeres on the five MIC chromosomes clustering together, respectively, which is also helpful to identify the midpoints of centromeres in the MIC. We revealed that the MAC chromosomes lack A/B compartments, topologically associating domains (TADs), and chromatin loops. The MIC chromosomes have TAD-like structures but not A/B compartments and chromatin loops. The boundaries of the TAD-like structures in the MIC are highly consistent with the chromatin breakage sequence (CBS) sites, suggesting that each TAD-like structure of the MIC chromosomes develops into one MAC chromosome during MAC development, which provides a mechanism of the formation of MAC chromosomes during conjugation. Overall, we demonstrated the distinct higher-order chromatin organization in the two nuclei of the T. thermophila and suggest that the higher-order chromatin structures may play important roles during the development of the MAC chromosomes.

    卷号:30

    期号:3

    页面范围:406-414

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