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M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{}~# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d.The ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # -$.%jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & /&0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ P3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WSIQMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 .z N07.8/jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & 90:1ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    9;2<3Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>0=4>5?6@7 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDEFGHIJK L M N O PQRSTUVWXr: gxY1ss( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]Q,!    wH       #&#+%xZC, .bsV ; o '0(#d T ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # -$.%jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & /&0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ P3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WSIQMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 .z N07.8/jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & 90:1ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    9;2<3Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>0=4>5?6@7 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDEFGHIJK L M N O PQRSTUVWX  0 ~p2(  p~ p s *,@ Z  @   p B@/@   @   p 64@ A pcn   J p C "A VGr17z7gH p 0޽h ? 3ff~___PPT10i.vו+D=' = @B +r4xY: xBY1Qs( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]Q,!    wH       #&#+%xZC, .bsV ; o '0(#d T ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u     %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # -$.%jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & /&0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ P3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WSIQMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 .z N07.8/jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & 90:1ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    9;2<3Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>0=4>5?6@7 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDEFGHIJK L M N O PQRSTUVWXr~ ZbY1h( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]7,!    wH       #&#+%xZC,  ; o ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ P3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WSIQMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 .z N0A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDHIJK L M N r c8 b Y1h( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]7,!    wH       #&#+%xZC,  ; o ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  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SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ P3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WSIQMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 .z N0A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDHIJK L M N rA, C5Y1h( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]7,!    wH       #&#+%xZC,  ; o ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ      !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxy{|}~<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  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W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ P3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WSIQMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 .z N0A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDHIJK L M N rw5 S5yY1Bi( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]7,!    wH       #&#+%xZC,  ; o ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ |3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WSIQMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 d. ehMQuRgl . N9 A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDHIJK L M N j  0 Px(  xr x S   H x 0޽h ? ̙33r i Y1i( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]7,!    wH       #&#+%xZC,  ; o ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ 3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 d. ehMQuRgl e. _AmcGrMQuRg f. WN~s|cvMQuRg . L9 A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDHIJK L M N j  0 Px(  xr x S   H x 0޽h ? ̙33r gs  tY1i( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]7,!    wH       #&#+%xZC,  ; o ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ 3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 d. ehMQuRgl e. _AmcGrMQuRg f. WN~s|cvMQuRg . L9 A8 ze PGWvgIQMQuRgl ^GWvgIQMQuRgl Sf[SIQMQuRg 5uSf[SIQMQuRg )/BCDHIJK L M N j  0 Px(  xr x S   H x 0޽h ? ̙33ru tIY1i( )   Chart MSGraph.Chart.80.Microsoft Graph 2000 Vh/ 0DTimes New RomanPPߡؖ2 0ؖD[SOes New RomanPPߡؖ2 0ؖ DArial BlackmanPPߡؖ2 0ؖ"0DArialBlackmanPPߡؖ2 0ؖ"@DWingdingskmanPPߡؖ2 0ؖPDўSOgdingskmanPPߡؖ2 0ؖ`DwiSO_GB2312kmanPPߡؖ2 0ؖ1pDSymbol12kmanPPߡؖ2 0ؖDN[_GB2312kmanPPߡؖ2 0ؖ1DIlA~ўS312kmanPPߡؖ2 0ؖw1DA~ўS312kmanPPߡؖ2 0ؖDJulius12kmanPPߡؖ2 0ؖwDEGGEurostileanPPߡؖ2 0ؖ@ .  @n?" dd@  @@``_@,WHOOSH.WAV.WAV 10103RIFFWAVEfmt ++data~~~~~~~~~~~~~~~~~~~~~~~~~~~|||~~~~~zvtvxz|~zvrnlrv||vtrpptz~|xvtv|~~zxvvvz|~xrlhntzzvrrpprrx~|j[QU_|bICCYn[ICY~zlnh]_r|]SUSSjz|x__l~v_]drnUb|nfd_]d]5=rrnj]jz~lMldrx[[_f|YQfWK_xh[zx[CIjzxxdSQz|_fI9WӹM;QnvK;OUx~xj]]YS~ɵlM?plM;CGpٵ[)Kh|% %;xtKQS]ɖbGr|lSnvz~nfS[nëx;Az=+AYݻ|=/1r_ ?|潄W5/Czãf)/pvbMQr|O=OtjdhjlG?G_ɊK3לdQCMppW;1SŷAMz/#?bx͖SAM[hvhYp~~zrvt[]ptWOUtxxd]v~vvxzd_xzh_nrSQltbdp~_lrQ_l][tf]W]~hWfp|fWhnhbU[hzlSCWbYbnxpdhjvjM9Ot]GQ[dpp~hhhdpx~xh]Yntnrp]dlvjQMb|vdjpzxzztljr||_[r~~jb_j|z|xpp~~v]Wh||zx|x|zzndhpxr]Yfntzjl|z||~vppnrzphrxz|~|xtrjjtv~~vz|z||xrrv~xrprtz|||vzxvx|xtx~~~~~~~zxxxx|zxz~~~rrz~|xz~~|vtx|zxz|~~~||zx|||~~zz|zz~~~~|~~||~~||zz|~||z~~~~zxzz|~~~~|~~~|~||~~~~~~~~~~~~~|||~~~~~~~~~~~~~||~~||~||~~~~|||~~~~||||~~~~~~||||~~~~~~~~~~~|~~~~|zz~~~~~~~||||~~||~~~~~|||~~~~~|||~|~|~~~~|z|~~~~~~~|||~~~~~~~~||~~~~~~~~ |tp]7,!    wH       #&#+%xZC,  ; o ?/R$+[8/fRb$9}JR25(cG[NRb$b^VWw1@dR${LVMVSzG C={b$\o"Nm6y L"$hSl%D)BsVR$8_/c)"&Ig 4hnR$mu5ʐ}7~B⭐qR$NzE;ف:h R$u|7h|(~SR$i(]|pH|H!R$kl[DM` OiR$l- s~V[wyHVzIR$۶`x=0JUR$\$cpAܻ([^kR$kޠ`㳆Yyb$P m86vb$As.2Kq Ə <AA@8&'uʚ;2Nʚ;g4TdTd@2 0zppp@ <4!d!d 0Pߡ<4dddd 0Pߡ <4BdBd8 0P(___PPT10D[SOes New RomanPPt2 0tDTimes New RomanPPt2 0t ppH___PPT9*nfFu88 PNG  IHDRW?sRGBgAMA7 cHRMz&u0`:pQ<e\'`MQuRgRIA BersonTYallow \MQuS^NRgSf[v~TRzN>e\'`MQuRgl0 w\vKm[ HQ\w\leQ)Yz SOQ [bdkbSvbSOAb Ag* + Ab === Ag*-Ab Km[NuNv@nh,g-Nw\vSm^ Ag + Ag*-Ab === Ag-Ab + Ag* ( + Ag*-Ab ) Km[Ag* S{h,g-N@b+T gvw\Ag Sm^  K  !     &7    4Rg[a Kmϑ+Tϑ_NOvuir;m'`STir Yˆ}v(v cSSO bSO o }>ueSTir 2urzo } ^o } oirS_uirI{0 OpNN'`:_ upOe^ؚ :p `$>e\'` TMO }S_c[NNvSOeP^ NN[[ gyrk2bb/g a$l؏(u5͑vppehVTՋBR b$3z['`- TMO }v[}Tv[w 4ZZ$  N vTMQuRgl    (uvhbSbbSO OvTMQulKm[/l } _ϑS^g  bTQIgGbSO TQb/l }bSO+/l }v~Tir +/l }7hT l g~Tvv~TirmS QTv^T[-NReQǏ'lS"lTN \O(uN[eT ~TTvv~Tir\ervNlS:N݄rvNir ReQ~bkmTr1u݄SĞ (ul450nmSlegSle"600nm vhNۏLhKm 8TIQe\'`alg hir N T V hKmel_N N T 6][$[  AgIQMQuRgl Fluonescense Immuuoassay 1gIQc 1 N,Bl a hKmg8^G0Rv7hT:N@n [ g_ؚvgIQ̀of0 @nˆ}v em 325-350 nm (ex 280 nm) @n-NNADHTƀ~ } 430-470 nm (ex 330-360 nm) cem > 500 nmvZ.".  M   &%   @ b. gIQcveXbKQeMOy 0 50 nm c. gIQcvid\8TIQ|pe'Y gIQNs^=\S0Wؚ d. gIQc3z[ NT~NbSObbS e N^_c[[Nv;m'` e. gIQcvoSl^MONSIQ:S V:NlYgIQBg(\ ex ^MONvis:S D$   &   f (2) g:gc gIQĞ 8^(uvc gIQNsؚ g}YvIQ3z['`TNOv)n^|pe0vQ:p/feXbKQeMOy\0 (Wx'`vagN N gIQĞNMQutˆ}vIgGv1u(lWwS^. W9Nf{|STir ^l(ueghbSO TgIQĞvk vQgIQNs NYgIQĞ S/fvQS\l 7hT̀ofr^pb\0dkY W9Nf{|N8^\O:NϑlyvcSSO0Z  FSFFJPM   (3) ё^\Tir gNzWё^\yP[ONgNMMOSOubOS:_gIQvMTir0 YEu(b!), Tb(b!), Nd(b!)N -NnMuir [-(lW2up NlNn Z(lov WIU >"xl'  ,  H2gIQMQuhKmlv{|W 1 GW(hKml GW(hKml NRyek el{O_ FO[8^S0Reg̀of@n vr^pb l|9hnc]hbSTcNbSOeSu]hbSgIQ'`(v9eS08^\GW(hlR:N gIQX:_l gIQsmpl csmpl gIQoSly 4ll gIQOP/cl<Zl6,    agIQX:_l ,glWNS_hirvbSTcNbSOeShvbSgIQ:_^X:_0 bgIQsmpl S_hvbSTc0RbSOegIQvϑP[Ns NM OY^'Y }vKm[ \PϑvbSOReQN]hT*ghv^'Y }mTir-N gIQsmp z^N^'Y }ϑvsQ0 .<)V  c. gIQoSly YgIQĞ\O:N~NSO W9Nf\O:NcSSO gIQĞvgIQNW9Nfv8T6eIQ1͑S TS8T6eMRvgIQ0 OTaUvKm[ i) (ugIQĞF hbS W9NfR hbSO Ag+AgF+AbR(Ag-AbR) + (AgF-AbR) AgvX[(WQ\NgIQvsmp z^ ii) bSOe/f~NSOS/fcSSO Ag + AbF + AbRAbF-Ag-AbRn#& H j2 ^GW(hKml ^GW(hKml(WhKmKNMR _{ۏLRy0 e8nyv]hvbS_{NTcNbSOvbS-NRyQeg b*gTcv]hvbSONbSTcvRRyQeg0vQRySǏy_lM bVvTcbSbbSO eg[s0 VvWhKml SbzN'`T^zN'`S^vhbSbbSO bSTcNZTirhb-----MQu8TDBR Xu 6",  eRgIQl md7hT̀ofr^pbvSNyel/feRgIQl07hT̀ofvgIQ[}TN,:N10ns g|̜TirvgIQ[}TN,:N10-100s0NeXbKQeMOy'Y _(uNeRlۏLKm[0 F \ dF1*!+"jeRgIQMQuStV Time-resolved Fluorescence Immunoassay r6# (" (# (# ((g  g  & ,# gIQMQuRglOp md>e\'` TMO }valg 3z['`}Y :p ؚv̀ofIQ P6RNupOe^ r^pbKm[   "  " $C ^BSf[SIQMQuRgl ُyRgele gSIQhKmvؚupOe^ S gMQuRglvyr_'`0 1;NSIQSO| s| _s|SvQMuir &qqZ IQl| TvUo 1 2-S'lWSTir Ǐ'lSIxo $kpkSIQSO| ~̃SIQSO|X6B0  2. SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^       !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ 3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 d. ehMQuRgl e. _AmcGrMQuRg f. WN~s|cvMQuRg . 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SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ      bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev cǏ'lSIxo Opup;m'` :pǏ'lSIxo4ln'`] gf4l0 N'lBgVCQsn ^vIQS\0NÒof0[v~'`V `o " " o!<HD x2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf Z\  ^/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ 3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 d. ehMQuRgl e. _AmcGrMQuRg f. 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SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ     bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev 0JJ"H 2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf d. x'`xxv ALP ^ZZ\  n/ &!Y:d. ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ 3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 d. ehMQuRgl e. _AmcGrMQuRg f. 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SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ     bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev 0JJ"H 2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf d. x'`xxv ALP ^ZZ\  n/ &!Y:ALP-AMPPD System% Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. 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SIQhir 1 SIQS^-Nmcvhir aLuminolSvQMuir LuminolvPTNMSOT vQSIQHesNO IsoluminolSvQMuir^l^(u Luminolv(lWSN[$MNO Isoluminolv(lWSN[$XR dk{|hS(uNTyRgir FOCLPSBR Vdk gؚv̀ofOS0 >ZZ     bTvUo{| NPSBR Op ؚϑP[Ns NÒofOSTfNNˆ}v(ޏc ;N(uNhJSbSTˆ}v(0 :p IQS\/fwev 0JJ"HZ; 2 NwSIQS^vhir ُ{|hir(WSIQǏ z-N Nm S^Ǐ z-NwPS\O(uvir(0 aǏ'lSirvperoxidase , POD Luminol + H2O2  (uPODhRgir bkgIQ }v ~̃gIQ }v vb,gf5 N3z[ gIQvWVv6R cgIQir(\O:Nhir gIQ } 9Ntp/l gIQ W9Nf d. x'`xxv ALP ^ZZ\  n/ &!Y:ALP-AMPPD System%Alkaline phosphatase (ALP) dephosphorylates AMPPD (1,2-N'lBgsNp{| to form a dioxetane anion, which fragments into adamantanone ёRpn and the excited state of methyl meta-oxybenzoate anion 'l2ux2uo4yP[ , the light emitter. The detect limit of ALP is 10-21mol/L. The derivatives of AMPPD are superior than the parent compound to the lighting rates and signal intensities. doxPP u    %   3 vhirGODTG-6-PDH )R(ugNv\O:Nhir 6qTǏhvvPSubvNir Q\O(uNSIQir( NNuuirSIQTSf[SIQ0 a| 4 VvlSIQMQuRg [ Ny_Yt d\O{O |nx^ؚ @b(uVv/ecir;N gZYNp6Rv{Tt @T":^ >7  zNlhg\RP[bSYǑ(u /TRSIQՋBR Ag+Ag-L+AbAg-Ab+Ab-Ag-L     hn ?!?    $P ! 9Y_lhg'YRP[bSYǑ(u Sp-Ab+AgSp-Ab-Ag Sp-Ab-Ag+Ab-LSp-Ab-Ag-Ab /TRSIQՋBR   hv vQ-N:L:NSIQhirbSIQ^ir, Ag:NbS, Ab:NbSO, Sp:NVv0 `9& `   &q, # 0'ACS 180H1u_Vb3lQSuN FUT T:NACS 180hQꁨRSf[SIQMQuRg|~ gACS 180PlusTACS 180sEI{WS,2000t^ScQe NvADVIACentaurMQuKm[|~ TvUoSǏSf[S^vchbSObbSS_(Wx'`N(-N'lSe ~ǏN*NN'lnv-NSO NuN*N5uoSv10N2uWTvUn S_[V Y0RW`eʑ>eQIQP[    91(2)Elesys (1010 2010 E-170)^tXWllQS(Roche)vElecsys ECLIA|~ ~TNTyHQۏb/g wQ gryrvO'` /fvMR(W;Sf[h-N^(uv/UNv5uSf[SIQMQuKm[NhVLL>03*4+5,6- 3 Sf[uirSIQMQuRglvyrp 1 Op ؚupOe^ [v~'`V Rg^_ ؚ b'` ؚ3z['` 9\ @b(uՋBRek md>e\'` TMO }valg0 &qZn,\ 2 :p [~gv͑ Y'`ebX[(WN[ SIQMQuKm[/fNywevSf[S^ MQuS^-NTyV }vr^pb 'lSS^-NTyV }vr^pb [PSS^St gv؏ NASRnZi [[vS^agN Nfc6R r^pbsa Nf0OTf0 `8+$~ 3 geۏU\ aYRgir|~vSU\ bAmRl\Rg(WMQuRg-Nv^ (u ORg^R_ (uN'Yϑ7h TvKm[0 c1uNSIQNvFUNS :RoNSf[S IQMQuRgv8^ĉ4N^^(uvSU\0 d. ehMQuRgl e. _AmcGrMQuRg f. 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