Associate professor
Supervisor of Master's Candidates
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DOI number:10.1016/j.jmps.2017.10.009
Journal:Journal of the Mechanics and Physics of Solids
Abstract:Quantitative characterizations of cell detachment are vital for understanding the fundamental mechanisms of cell adhesion. Experiments have found that cell detachment shows strong rate dependence, which is mostly attributed to the binding-unbinding kinetics of receptor-ligand bonds. However, our recent study showed that cellular volume regulation can significantly regulate the dynamics of adherent cells and cell detachment. How this cellular volume regulation contributes to the rate dependence of cell detachment remains elusive. Here, we systematically study the role of cellular volume regulation in the rate dependence of cell detachment by investigating the cell detachments of nonspecific adhesion and specific adhesion. We find that the cellular volume regulation and the bond kinetics dominate the rate dependence of cell detachment at different time scales. We further test the validity of the traditional Johnson–Kendall–Roberts (JKR) contact model and the detachment model developed by Wyart and Gennes et al (W–G model). When the cell volume is changeable, the JKR model is not appropriate for both the detachments of convex cells and concave cells. The W–G model is valid for the detachment of convex cells but is no longer applicable for the detachment of concave cells. Finally, we show that the rupture force of adherent cells is also highly sensitive to substrate stiffness since an increase in substrate stiffness will lead to more associated bonds. These findings can provide insight into the critical role of cell volume in cell detachment and might have profound implications for other adhesion-related physiological processes.
First Author:Yuehua Yang
Indexed by:Journal paper
Correspondence Author:Hongyuan Jiang
Volume:112
Page Number:594-618
Translation or Not:no
Date of Publication:2018-03-01
Included Journals:SCI
Links to published journals:https://linkinghub.elsevier.com/retrieve/pii/S0022509617306609
