Chemiluminescent Fe3O4@Nickel–Cobalt Double Hydroxide Magnetic Core–Shell Nanomaterial as an Analytical Interface for Label-Free CYFRA21-1 Immunosensing

Release time:2024-11-18  Hits:

• DOI number:10.1021/acs.analchem.4c05038
• Affiliation of Author(s):化学与材料科学学院
• Journal:Anal. Chem.
• Abstract:Cytokeratin 19 fragment (CYFRA21-1) is considered to be a potential marker for the diagnosis and of classification of lung cancer. It is highly desired to develop rapid and highly sensitive label-free chemiluminescence (CL) immunosensors for the detection of CYFRA21-1. In this work, magnetic core–shell nanocomposite Fe3O4@nickel–cobalt double hydroxide (Fe3O4@DH) was used for the first time as a carrier to synthesize N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and gold and silver nanocluster (AuAgNC) bifunctionalized magnetic nanomaterials Fe3O4@DH/AuAgNCs-ABEI. The resulting Fe3O4@DH/AuAgNCs-ABEI demonstrated excellent CL performance, attributed to the exceptional catalytic capability of nickel–cobalt double hydroxide (NiCo-DH) and AuAgNCs, as well as the large specific surface area of NiCo-DH for AuAgNCs and ABEI enrichment. Moreover, Fe3O4@DH/AuAgNCs-ABEI exhibited superior stability compared to Fe3O4-based CL-functionalized magnetic nanomaterials, owing to the presence of NiCo-DH shells. On this basis, a label-free CL immunosensor using CYFRA21-1 antibody-modified Fe3O4@DH/AuAgNCs-ABEI as an analytical interface was constructed for the detection of CYFRA21-1 in the range from 1.0 × 10–13 to 1.0 × 10–8 g/mL. The detection limit of this CL immunosensor was 47.7 fg/mL, 4 orders of magnitude lower than existing CL methods. The CL immunosensor was able to accurately detect the concentration of CYFRA21-1 in serum samples, as evidenced by ELISA results. More importantly, it not only distinguished well between healthy persons and lung cancer patients (90.0% sensitivity and 90.0% specificity), but also effectively distinguished between patients with non-small cell lung cancer and small cell lung cancer (80.0% sensitivity and 86.7% specificity).
• Co-author:张婼娴,杨donglian
• First Author:王慢莉
• Indexed by:Journal paper
• Correspondence Author:Teng Gang,shujiangnan,Hua Cui
• Discipline:Natural Science
• Document Type:J
• Translation or Not:no
• Date of Publication:2024-11-13
• Included Journals:SCI
• Links to published journals:https://pubs.acs.org/doi/full/10.1021/acs.analchem.4c05038