yangxinxing
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- Supervisor of Doctorate Candidates
- Supervisor of Master's Candidates
- Name (English):Xinxing Yang
- Name (Pinyin):yangxinxing
- E-Mail:
- Administrative Position:Principal investigator
- Education Level:With Certificate of Graduation for Doctorate Study
- Business Address:西区医算楼807
- Contact Information:xinxingyang@ustc.edu.cn
- Degree:Dr
- Alma Mater:Peking Univeristy
- Teacher College:Life Sciences
- Discipline:Biology
Contact Information
- ZipCode：
- PostalAddress：
- Email：
- Paper Publications
Protocol for single-molecule labeling and tracking of bacterial cell division proteins
Release time:2024-01-21 Hits:
- DOI number:10.1016/j.xpro.2023.102766
- Journal:STAR protocols
- Key Words:Single-molecule Assays; Microbiology; Microscopy
- Abstract:Here, we present a protocol for labeling and tracking individual molecules, particularly cell division proteins in live bacterial cells. The protocol encompasses strain construction, single-molecule imaging, trajectory segmentation, and motion property analysis. The protocol enables the identification of distinctive motion states associated with different cell division proteins. Subsequent assessments of the dynamic behaviors of these proteins provide insights into their activities and interactions at the septum during cell division. For complete details on the use and execution of this protocol, please refer to Yang et al. (2021),1 Lyu et al. (2022),2 and Mahone et al. (2024).3
- Co-author:Jinchan Xue
- First Author:Di Yan
- Indexed by:Journal paper
- Correspondence Author:Jie Xiao,Zhixin Lyu,Xinxing Yang
- Discipline:Natural Science
- Document Type:J
- Translation or Not:no
- Date of Publication:2023-12-12
- Links to published journals:https://www.sciencedirect.com/science/article/pii/S2666166723007335
