主要研究领域：

真核细胞中RNA的表达与加工的调节，真核生物中转录调节机制，非编码RNA的表达与调节机制，模式生物的遗传与发育，以及基因编辑和染色体操纵的新技术和新方法。

 

 

主要研究成果：

    发现真核生物中无内含子的基因含有一些顺式作用的RNA序列元素，通过“绑架”细胞内正常基因表达和加工的所需因子，提高无内含子基因mRNA的稳定性，poly(A)聚腺苷酸化，以及从细胞核到细胞质的运输(NAR 2005; MCB 2005)。确认了高等动物中存在着细胞核里的基因干扰现象；发现了siRNA从细胞质转运到细胞核的途径；发现和解析了nrde 通路；发现细胞核RNA干扰可以抑制RNA聚合酶II的转录延伸，造成转录的提前终止；发现高等动物中siRNA造成组蛋白的H3K9me3和H3K27me3甲基化(Science, 2008；Nature, 2010；PLoS Genetics，2011；Genetics, 2014；Curr. Biol., 2015)；发现细胞核和细胞质RNAi干扰通路都参与了生物体获得性性状的多代遗传(Nature Genetics，2012；Cell Reports, 2018）；发现了核仁RNA 干扰，发现了一类受环境刺激调控的针对rRNA的小干扰RNA(risiRNA)和抑制risiRNA产生的Susi通路，发现了一种新的rRNA稳态调节机制(Nat Struct Mol Biol, 2017；PNAS, 2018；Nucleic Acids Research, 2021a)；发现和解析了介导piRNA转录起始的USTC复合物和异染色质识别蛋白以及帮助piRNA转录后加工的PICS复合物(Genes & Development, 2019；Cell Reports, 2019；PNAS, 2021；Nature Communications, 2021；JGG, 2022)；发现新的组蛋白修饰和识别蛋白以及调控寿命的新机制(Elife 2022)；发展了基因编辑的新方法 (Sci. Rep., 2014；Genetics,2015; G3, 2018）。

代表性学术论文：

Meng Huang(#), Minjie Hong(#), Chengming Zhu, Di Chen(*), Xiangyang Chen(*), Shouhong Guang(*), and Xuezhu Feng(*) (2022) H3K9me1/2 methylation limits the lifespan of daf-2 mutants in C. elegans. Elife. 2022 Sep 20;11:e74812. doi: 10.7554/eLife.74812.

 

Xuelian Ma(#), Shumin Cheng(#), Ruofan Ding, Zhaozhao Zhao, XuDong Zou, Shouhong Guang, Qixuan Wang, Huan Jing, Chen Yu, Ting Ni, Lei Li(*) (2022)  ipaQTL-atlas: an atlas of intronic polyadenylation quantitative trait loci across human tissues. Nucleic Acids Research, 2022 Aug 31, https://doi.org/10.1093/nar/gkac736

 

Xinhao Hou(#), Chengming Zhu, Mingjing Xu, Xiangyang Chen, Cheng Sun, Björn Nashana(*), Shouhong Guang(*), and Xuezhu Feng(*) (2022) The SNAPc complex mediates starvation-induced trans-splicing in C. elegans. Journal of Genetics and Genomics, 2022 Mar 10:S1673-8527(22)00076-5. doi: 10.1016/j.jgg.2022.02.024.

 

Xiaoyang Wang(#), Chenming Zeng(#), Shanhui Liao(#), Zhongliang Zhu, Jiahai Zhang, Xiaoming Tu, Xuebiao Yao, Xuezhu Feng(*), Shouhong Guang(*), and Chao Xu(*) (2021) Molecular basis for PICS-mediated piRNA biogenesis and cell division. Nature Communications, 2021 Sep 22;12(1):5595. doi: 10.1038/s41467-021-25896-7

 

Shimiao Liao(#), Xiangyang Chen(#), Ting Xu(#), Qile Jin, Zongxiu Xu, Demin Xu, Xufei Zhou, Chengming Zhu(*), Shouhong Guang(*) and Xuezhu Feng(*) (2021a) Antisense ribosomal siRNAs inhibit RNA polymerase I-directed transcription in C. elegans Nucleic Acids Research 2021 Sep 20;49(16):9194-9210. doi: 10.1093/nar/gkab662.

 

Zheng Xu(#), Jie Zhao, Minjie Hong, Chenming Zeng, Shouhong Guang(*), Yunyu Shi(*) (2021b) Structural recognition of the mRNA 3’ UTR by PUF-8 restricts the lifespan of C. elegans. Nucleic Acids Research, 2021 Sep 27;49(17):10082-10097. doi: 10.1093/nar/gkab754

 

Xinya Huang(#), Peng Cheng(#), Chenchun Weng, Zongxiu Xu, Chenming Zeng, Xiangyang Chen(*), Chengming Zhu(*), Shouhong Guang(*), and Xuezhu Feng(*) (2021) A chromodomain protein mediates heterochromatin-directed piRNA expression. PNAS, 2021 Jul 6;118(27):e2103723118. doi: 10.1073/pnas.2103723118

 

Chenming Zeng (#), Chenchun Weng (#), Xiaoyang Wang (#), Yong-Hong Yan (#), Wen-Jun Li, Demin Xu, Minjie Hong, Shanhui Liao, Meng-Qiu Dong, Xuezhu Feng (*), Chao Xu (*), and Shouhong Guang (*) (2019) Functional proteomics identifies a PICS complex required for piRNA maturation and chromosome segregation. Cell Reports 2019 Jun 18;27(12):3561-3572.e3. doi: 10.1016/j.celrep.2019.05.076

 

Chenchun Weng (#), Joanna Kosalka (#), Ahmet C. Berkyurek (#), Przemyslaw Stempor, Xuezhu Feng, Hui Mao, Chenming Zeng, Wen-Jun Li, Yong-Hong Yan, Meng-Qiu Dong, Natalia Rosalía Morero, Cecilia Zuliani, Orsolya Barabas, Julie Ahringer, Shouhong Guang (*), and Eric A. Miska (*) (2019) The USTC complex co-opts an ancient machinery to drive piRNA transcription in C. elegans. Genes & Development 2019 Jan 1;33(1-2):90-102. doi: 10.1101/gad.319293.118. Epub 2018 Dec 19.

 

Chengming Zhu (#), Qi Yan (#), Chenchun Weng, Xinhao Hou, Hui Mao, Dun Liu, Xuezhu Feng (*), Shouhong Guang (*) (2018) Erroneous ribosomal RNAs promote the generation of antisense ribosomal siRNA. PNAS 2018 Oct 2;115(40):10082-10087. doi: 10.1073/pnas.1800974115.

 

Chen X (#*), Liao S (#), Huang X, Xu T, Feng X, Guang Shouhong (*). (2018) Targeted Chromosomal Rearrangements via a Combinatorial Use of CRISPR/Cas9 and Cre/LoxP Technologies in Caenorhabditis elegans. G3 (Bethesda). 2018 Jul 31;8(8):2697-2707. doi: 10.1534/g3.118.200473.

 

Fei Xu (#), Xuezhu Feng (#), Xiangyang Chen, Chenchun Weng, Qi Yan, Ting Xu, Minjie Hong, and Shouhong Guang (*)（2018）A cytoplasmic Argonaute protein promotes the inheritance of RNAi. Cell Reports.2018 May 22;23(8):2482-2494. doi: 10.1016/j.celrep.2018.04.072

 

Zhou X (#), Chen X (#), Wang Y, Feng X (*), Shouhong Guang (*). (2017) A new layer of rRNA regulation by small interference RNAs and the nuclear RNAi pathway. RNA Biol. 2017 Nov 2;14(11):1492-1498. doi: 10.1080/15476286.2017.1341034. [Epub ahead of print]

 

Xufei Zhou (#), Xuezhu Feng (#), Hui Mao, Mu Li, Fei Xu, Kai Hu, and Shouhong Guang(*)  (2017) RdRP-synthesized antisense ribosomal siRNAs silence pre-rRNA via the nuclear RNAi pathway. Nature Structural & Molecular Biology 2017 Mar;24(3):258-269. doi: 10.1038/nsmb.3376.

 

Li G, Wu X, Qian W, Cai H, Sun X, Zhang W, Tan S, Wu Z, Qian P, Ding K, Lu X, Zhang X, Yan H, Song H, Guang Shouhong, Wu Q, Lobie PE, Shan G, Zhu T.(2016) CCAR1 5' UTR as a natural miRancer of miR-1254 overrides tamoxifen resistance. Cell Research 2016 Jun;26(6):655-73. doi: 10.1038/cr.2016.32.

 

Chen X (#), Li M (#), Feng X (*), Shouhong Guang (*) (2015) Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans. Genetics 2015 Dec;201(4):1295-306 doi: 10.1534/genetics.115.181883.

 

Hui Mao (#), Chengming Zhu (#), Dandan Zong, Chenchun Weng, Xiangwei Yang, Hui Huang, Dun Liu, Xuezhu Feng (*), and Shouhong Guang (*) (2015) The Nrde pathway mediates small RNA-directed histone H3 lysine 27 trimethylation in Caenorhabditis elegans. Current Biology 2015 Sep 21;25(18):2398-403. doi: 10.1016/j.cub.2015.07.051. Epub 2015 Sep 10.

 

Xiangyang Chen (#), Fei Xu (#), Chengming Zhu, Jiaojiao Ji, Xufei Zhou, Xuezhu Feng (*), and Shouhong Guang (*) (2014) Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans. Scientific Reports 2014 Dec 22;4:7581. doi: 10.1038/srep07581.

 

Zhou X (#), Xu F (#), Mao H, Ji J, Yin M, Feng X (*), and Shouhong Guang (*) (2014) Nuclear RNAi Contributes to the Silencing of Off-Target Genes and Repetitive Sequences in Caenorhabditis elegans. Genetics 2014 May;197(1):121-32. doi: 10.1534/genetics.113.159780.

 

Xuezhu Feng and Shouhong Guang(*) (2013) Small RNAs, RNAi and the Inheritance of Gene Silencing in Caenorhabditis elegans. Journal of Genetics and Genomics2013，Apr 20;40(4):153-60. doi: 10.1016/j.jgg.2012.12.007. Epub 2013 Feb 4. Review.

 

Sam Guoping Gu, Julia Pak, Shouhong Guang, Jay M. Maniar, Scott Kennedy, and Andrew Fire, (2012) Amplification of siRNA in Caenorhabditis elegans generates a transgenerational sequence-targeted histone H3 lysine 9 methylation footprint. Nature Genetics 2012，Jan 8;44(2):157-64. doi: 10.1038/ng.1039..

 

Burkhart, K.B., Guang, Shouhong, Bochner, A.F., and Kennedy, S., (2011) A pre-mRNA–associating factor links endogenous siRNAs to chromatin regulation. PLoS Genetics,2011 Aug;7(8):e1002249. doi: 10.1371/journal.pgen.1002249. Epub 2011 Aug 25..

 

Guang, Shouhong (#), Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Burton, N., and Kennedy, S., (2010) Small regulatory RNAs inhibit RNA Polymerase II during the elongation phase of transcription. Nature, 2010，Jun 24;465(7301):1097-101. doi: 10.1038/nature09095. Epub 2010 Jun 13.

 

Guang, Shouhong (#), Bochner, A.F. (#), Pavelec, D.M., Burkhart, K.B., Harding, S., Lachowiec, J., and Kennedy, S., (2008) An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science Jul 25;321(5888):537-41. doi: 10.1126/science.1157647. Erratum in: Science. 2009 Dec         

 

Guang, Shouhong, Felthauser, A., and Mertz, J. (2005) Binding of hnRNP L to the pre-mRNA processing enhancer (PPE) of herpes simplex virus’ thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs. Mol. Cell. Biol.Aug;25(15):6303-13.

 

Guang, Shouhong and Mertz, J.E. (2005) PPE-like elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes. Nucleic Acid Res. Apr 20;33(7):2215-26. Print 2005.