NicE-C technique for detecting chromatin interactions between chromatin open sites
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Disigner of the Invention:Xiaoyuan Song,Zhang Ran,luozhengyu,hutengfei
Patent description:本发明提供了检测染色质开放位点间染色质相互作用的NicE-C方法,其参考了NicE-seq与Hi-C技术 ,在前期用NicE-seq技术中使用的Nt.CivPH和DNA聚合酶I这两个酶替代Hi-C技术中使用的限制性内切酶进行染色质切割,从而实现对染色质开放位点的切割。后续参考Hi-C技术
等在末端补平(不需在此处加入生物素标记),加dA尾后通过生物素标记的桥接头连接来实现对染色质相互作用的捕获。本发明具有样品起始细
胞量少,操作简便,信号富集效果好,分辨率高等特点。
Type of Patent:Invent
State of Patent:Pending patent
Application Number:202110773743.2
Number of Inventors:4
Service Invention or Not:no
Application Date:2021-07-08
Publication Date:2021-10-22
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Attachments:
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NicE-C 专利_p1.pdf
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