实验室长期聚焦于单分子超分辨显微成像与空间多组学技术的开发及神经生物学应用，取得了包括突触纳米柱在内的重要发现，成果发表于 Nature、Nature Methods、Nature Neuroscience 等期刊。

当前研究聚焦两大方向：

一、利用MINFLUX等超分辨技术解析突触纳米结构-功能关系及其在疾病中的异常机制；

二、研发空间多组学成像技术，可在厘米级脑片上实现上千基因的亚细胞分辨率解析，并搭建了集全自动成像与智能分析于一体的MetaScope平台。

通过整合纳米动态成像与大尺度空间组学，实验室构建了跨尺度成像工具链，为神经系统研究与疾病诊疗提供关键技术支撑。

Publications (Selected from 49)   * Co-first author, ^# Corresponding author

1.     Lin J*, Chen C*, Li S^#, Chen L, Fang M, Fu S, Chen K, Xu H, Zhang F, Wang R, Jiang C, Tang M, Liu Z, Wang S, Liu K, Niu C, Li B, Liu X, Cheng C^#, Tang AH^#, Qu K^# (2026) Multi-omics analysis characterizes the spatial architecture of glioblastoma ecosystems. Nat Neurosci. In press.

2.     Ke W, Lyu S, Jin M, Jiao S, Li L, Zhang H, Ling K, Tian C, Song Y, Chen L, He O, Xiao YJ, Deng S, Fan L, Mu H, Yang H, Xu H, Wang H, Tang A, Peng J, He J, Guo H, Shu Y (2026) Spindle neurons in human cortex possess distinctive firing properties and transcriptomic signatures. Nat Commun. In Press.

3.  Chen L, Teng X^#, Tang AH^# (2026) GLASS-seq: a gel-anchored, ligation-assisted, scalable biosensing platform for low-cost regional spatial transcriptomics. Biosens. Bioelectron. 311: 118973.

4.     Lin JK, Liu Y, Deng J, Wang HL, Li Q, Chen L, Ding WQ, Tang AH, Dou YN^#, Sun YG^# (2026) Transcriptomic and spatial organization of mouse spinal cord projection neurons. Cell Rep. 45(1):116717.

5.     Gu W*, Chen JH*, Zhang Y*, Wang Z*, Li J*, Wang S, Zhang H, Jiang A, Zhong Z, Zhang J, Xu Z, Liu P, Xi C, Hou T, Gill DL, Li D, Mu Y^#, Wang SQ^#, Tang AH^#, Wang Y^# (2025) Highly dynamic and sensitive NEMOer calcium indicators for imaging ER calcium signals in excitable cells. Nat Commun. 16, 3472.

6.     Xu N*^#, Chen SY*, Tang AH^# (2025) Tuning synapse strength by nanocolumn plasticity. Trends Neurosci. 48(3):200-212.

7.     Yan HH, He JJ, Fu C, Chen JH^#, Tang AH^# (2025) ATAD1 regulates neuronal development and synapse formation through tuning mitochondrial function. Int. J. Mol. Sci. 26(1):44.

8.     Sun SY, Nie LY, Fang X, Luo HM, Fu C^#, Wei Z^#, Tang AH^# (2025) The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons. Neural Regen Res. 20(1):209-223.

9.     Chen JH^#, Tang AH^# (2024) Nanoscale reorganization of glutamate receptors underlies synaptic plasticity and pathology. Neurosci Bull. 40:840–844.

10.     Xu N*, Cao R*, Chen SY, Gou XZ, Wang B, Luo HM, Gao F, Tang AH (2024) Structural and functional reorganization of inhibitory synapses by activity-dependent cleavage of neuroligin-2. Proc. Natl. Acad. Sci. U.S.A., 121(18): e2314541121.

11.  Chen JH*^#, Xu N*, Qi L, Yan HH, Wan FY, Gao, F, Fu C, Cang C, Lu B, Bi GQ, Tang AH^# (2023) Reduced lysosomal density in neuronal dendrites mediates deficits in synaptic plasticity in Huntington’s disease. Cell Rep, 42(12): 113573.

12.  He J, Liu K, Wu Y, Zhao C, Yan S, Chen JH, Hu L, Wang D, Zheng F, Wei W, Xu C, Huang C, Liu X, Yao X, Ding L, Fang Z#, Tang AH^#, Fu C^# (2023) The AAA-ATPase Yta4/ATAD1 interacts with Dnm1 and Fis1 to inhibit mitochondrial fission.  Plos Bio., 21(8): e3002247.

13.  Li D, Cao R, Li Q, Yang Y, Tang AH, Zhang J, Liu Q (2023) Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment. Nat Struct Mol Biol, 30(7):914-925.

14.  Li J*, Shang Z*, Chen JH* , Gu W*, Yao L*, Yang X, Sun X, Wang L, Wang T, Liu S, Li J, Hou T, Xing D, Gill DL, Li J, Wang SQ, Hou L, Zhou Y^#, Tang AH^#, Zhang X^#, Wang Y^# (2023) Engineering of NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods, 20: 918–924.

15.  Zhu WH, Yang XX, Gou XZ, Fu SM, Chen JH, Gao F, Shen Y, Bi D^#, Tang, AH^# (2023) Nanoscale reorganisation of synaptic proteins in Alzheimer's disease. Neuropathol. Appl. Neurobiol. 49(4):e12924.

16.  Han Y*, Cao R*, Qin L, Chen LY, Tang AH^#, Südhof TC^#, Zhang B^# (2022) Neuroligin3 confines AMPA receptors in nanoclusters and controls synaptic strength at the calyx of Held. Sci Adv., 8(24): eabo4173.

17.  Gou XZ*, Ramsey AM*^#, Tang AH^# (2022) Re-examination of the determinants of synaptic strength from the perspective of superresolution imaging. Curr Opin Neurobiol, 74:102540.

18. Ramsey A*, Tang AH*, LeGates TA, Gou XZ, Carbone BE, Thompson SM,  Biederer T and Blanpied TA (2021) Subsynaptic positioning of AMPARs by LRRTM2 controls synaptic strength. Sci Adv. 7(34): eabf3126.

19.  Tang AH*^#, Chen H*, Li TP, Metzbower SR, Mac Gillavry HD and Blanpied TA^# (2016) A transsynaptic nanocolumn aligns neurotransmitter release to receptors. Nature. 536: 210–214.